Distilled water and 95% ethanol were used to make a 20% ethanol solution that was self-administered orally in the home cage during the two-bottle choice procedure and in the operant chamber. During surgery, osmotic mini-pumps were filled with 2 mL of drug solution. The drug solution used for the Sal group was 0.9% sterile saline solution. The drug solution for the Nic group was made using nicotine hydrogen tartrate salt (MP Biomedicals, LLC.) dissolved in 0.9% sterile saline solution. Nicotine was delivered at approximately 3 mg/kg/day [12 (link)].
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