VACV Infection in Cell Lines

African green monkey kidney epithelioid cells (BS-C-1), and human cervix carcinoma epithelioid cells (HeLa) were grown in Dulbecco׳s modified Eagle׳s medium (DMEM) (Life Technologies) containing 50 IU/ml penicillin, 50 μg/ml streptomycin (Sigma) and 10% foetal bovine serum (FBS) (Life Technologies). Cells were incubated at 37 °C in a 5% CO₂ incubator. VACV strain Western Reserve (VACV) and VACV-A5L-EGFP (Carter et al., 2003 (link)) were used in this work. The anti-A46 antibody was a kind gift from Andrew Bowie (Stack et al., 2005 (link)), the anti-D8 antibody a kind gift from Geoffrey Smith (Parkinson and Smith, 1994 (link)). Other antibodies used were anti-RAB1A (ab97956, Abcam), anti-GM130 (610822, BD Pharmingen), and anti-B5 (NR-556, BeiResources).