Lung Cancer Tissue Analysis Protocol

Human lung cancer tissue microarray slides with matched metastatic lymph node were purchased from US Biomax (LC814a and LC817). Immunohistochemistry and histopathology analyses as well as immunohistochemical scoring were performed in the same manner as described previously [5 (link), 6 (link)]. Scores were generated by the percentage of positive cells multiplied by stain intensity (score 0 = negative, 1-2 = weak, 3-4 = moderate, 5-6 = strong). The images were scanned into a digital format by Scanscope XT system (Aperio Technologies, Vista, CA) and analyzed using Aperio ImageScope 9.1 software (Aperio Technologies).

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Ho M.Y., Liang C.M, & Liang S.M. (2016). PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-κB signaling in lung cancer. Oncotarget, 7(32), 52255-52269.

Publication 2016

LC814a LC817 Scanscope XT system Aperio ImageScope 9.1 software

Cells Human Immunohistochemistry Lung cancer Lymph node Microarray Stain Tissue Weak

Corresponding Organization :

Other organizations : Genomics Research Center, Academia Sinica, Agricultural Biotechnology Research Center, Academia Sinica

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Variable analysis

independent variables

Lung cancer tissue microarray slides
Metastatic lymph node

dependent variables

Immunohistochemistry and histopathology analyses
Immunohistochemical scoring

control variables

Immunohistochemistry and histopathology analyses were performed in the same manner as described previously [5, 6]
Immunohistochemical scoring was performed using a standardized scoring system (score 0 = negative, 1-2 = weak, 3-4 = moderate, 5-6 = strong)

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