Whole-body composition was measured using a Hologic QDR 4500A DXA machine and analysed using Apex software version 4.0.2 (Hologic Inc., Bedford, USA). The machine was calibrated daily using a phantom spine, and coefficients of variation during the course of the study were <2% for total fat mass. All standard DXA measurements were analysed using Hologic APEX 3.1 software (Hologic). The APEX software defines gluteofemoral fat (gynoid fat) as lying in a region from the head of the femur to the mid-thigh, while leg fat was measured in a region demarcated by an oblique line passing through the femoral neck and joining a central vertical line between the legs (see Supplementary Fig. 1, see section on supplementary materials given at the end of this article). Whole-body (excluding head) fat mass was recorded. Abdominal visceral adipose tissue and s.c. adipose tissue were measured according to previously described methodology (17 (link)).
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Whole-body Composition Analysis via DXA
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Abdominal adipose tissue
Adipose tissue
Body
Body composition
Central line
Femoral neck
Head
Head of the femur
Legs
Spine
Thigh
Corresponding Organization :
Other organizations : University of the Witwatersrand, National Health Laboratory Service, South African Medical Research Council, University of Southampton
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Variable analysis
independent variables
- None explicitly mentioned
- Whole-body (excluding head) fat mass
- Abdominal visceral adipose tissue
- Subcutaneous adipose tissue
- Calibration of Hologic QDR 4500A DXA machine daily using a phantom spine
- Coefficients of variation during the course of the study were <2% for total fat mass
- Positive control: Phantom spine used for daily calibration of the DXA machine
- Negative control: Not mentioned
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