Conditional Knockout of Stat3 in CD11c+ Cells

C57BL/6J mice were acquired from The Jackson Laboratory. B6.Cg-Tg (Itgax-cre)1-1Reiz/J mice (CD11c-Cre; 008068; The Jackson Laboratory) were bred with Stat3^f/f mice (Caton et al., 2007 (link); Takeda et al., 1998 (link)) to generate CD11c-Cre⁺Stat3^f/f mice (Stat3^Δ/Δ) and Cre-negative Stat3^f/f littermate controls (termed herein, Stat3^+/+). All mice were maintained in a specific pathogen–free animal facility at MD Anderson Cancer Center. Mice of both sexes were analyzed, ranging in age from 7 through 13 wk. Age- and sex-matched littermates were chosen randomly for inclusion in treatment groups and were used in accordance with MD Anderson Cancer Center Institutional Animal Care and Use Committee–approved protocols.

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Zhou Y., Medik Y.B., Patel B., Zamler D.B., Chen S., Chapman T., Schneider S., Park E.M., Babcock R.L., Chrisikos T.T., Kahn L.M., Dyevoich A.M., Pineda J.E., Wong M.C., Mishra A.K., Cass S.H., Cogdill A.P., Johnson D.H., Johnson S.B., Wani K., Ledesma D.A., Hudgens C.W., Wang J., Wadud Khan M.A., Peterson C.B., Joon A.Y., Peng W., Li H.S., Arora R., Tang X., Raso M.G., Zhang X., Foo W.C., Tetzlaff M.T., Diehl G.E., Clise-Dwyer K., Whitley E.M., Gubin M.M., Allison J.P., Hwu P., Ajami N.J., Diab A., Wargo J.A, & Watowich S.S. (2022). Intestinal toxicity to CTLA-4 blockade driven by IL-6 and myeloid infiltration. The Journal of Experimental Medicine, 220(2), e20221333.

Publication 2022

C57BL/6J mice B6.Cg-Tg (Itgax-cre)1-1Reiz/J mice CD11c-Cre;

Animal C57bl mice Cancer Institutional animal care and use committee Mice Specific pathogen free Stat3

Corresponding Organization : The University of Texas Health Science Center at Houston

Other organizations : Tsinghua University, Memorial Sloan Kettering Cancer Center, Parker Institute for Cancer Immunotherapy

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Variable analysis

independent variables

Genotype: CD11c-Cre+Stat3^f/f (Stat3^Δ/Δ) and Cre-negative Stat3^f/f (Stat3^+/+) mice

dependent variables

Not explicitly mentioned

control variables

Mouse strain: C57BL/6J mice
Age: 7-13 weeks
Sex: both males and females
Maintenance: specific pathogen-free animal facility at MD Anderson Cancer Center

positive controls

None specified

negative controls

Cre-negative Stat3^f/f (Stat3^+/+) littermate controls

Annotations

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