Establishing Cell Lines for Meningioma Research

Ben-Men-1 benign meningioma cells have been described (15 (link)). Malignant meningioma KT21-MG1 and IOMM-Lee cells (8 (link),9 (link)) were kindly provided by Dr. Anita Lal, University of California, San Francisco. HE1-1 is an adenovirus E1-transformed human embryonic kidney cell line (unpublished), and HMS-97 is a human malignant schwannoma cell line (26 (link)). All cell lines and primary human meningeal cells (ScienCell) were grown in Dulbecco’s modified eagle medium with 10% fetal bovine serum (Invitrogen). AR-42 and AR-12 were supplied by Arno Therapeutics and formulated into rodent chow (Research Diets) to deliver about 25mg/kg/day and 100mg/kg/day, respectively (21 (link)–23 (link)). Also, AR-42 was dissolved in dimethylsulfoxide (DMSO) for in vitro experiments.

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Burns S.S., Akhmametyeva E.M., Oblinger J.L., Bush M.L., Huang J., Senner V., Chen C.S., Jacob A., Welling D.B, & Chang L.S. (2012). Histone deacetylase inhibitor AR-42 differentially affects cell cycle transit in meningeal and meningioma cells, potently inhibiting NF2-deficient meningioma growth. Cancer research, 73(2), 792-803.

Publication 2012

Adenovirus Ar 12 Ar 42 Benign meningioma Cell lines Cells Diets Dimethylsulfoxide Eagle Embryonic Fetal bovine serum Human Kidney Malignant meningioma Malignant schwannoma Men 1 Meningeal Rodent Therapeutics Transformed cell line

Corresponding Organization : University Hospital Münster

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Variable analysis

independent variables

Concentration of AR-42 (about 25mg/kg/day and 100mg/kg/day)
Formulation of AR-42 and AR-12 into rodent chow

dependent variables

Cell growth and proliferation of cell lines (Ben-Men-1 benign meningioma, KT21-MG1 and IOMM-Lee malignant meningioma, HE1-1 adenovirus E1-transformed human embryonic kidney, and HMS-97 human malignant schwannoma)

control variables

Cell culture medium (Dulbecco's modified eagle medium with 10% fetal bovine serum)
Cell incubation conditions (not explicitly mentioned)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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