Multiplex Cytokine Profiling in Cell Culture

The supernatants from each of the wells of the cell culture experiments were collected and transferred into a 96-well plate using Biolegend LEGENDplex Human CD8/NK Panel (13-plex, Catalog#740267) according to the manufacturer’s instructions. Samples were run in BD LSRFortessa Flow cytometer and results were acquired using Legendplex v8.0 software (Biolegend, San Diego, CA, USA) [24 (link)].

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Zhang Z., Yang A., Chaurasiya S., Park A.K., Lu J., Kim S.I., Warner S.G., Yuan Y.C., Liu Z., Han H., Von Hoff D., Fong Y, & Woo Y. (2021). CF33-hNIS-antiPDL1 Virus Primes Pancreatic Ductal Adenocarcinoma for Enhanced anti-PD-L1 Therapy. Cancer gene therapy, 29(6), 722-733.

Publication 2021

LEGENDplex Human CD8/NK Panel (13-plex, Legendplex v8.0 software

Cell culture Human

Corresponding Organization :

Other organizations : City Of Hope National Medical Center, Translational Genomics Research Institute

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Variable analysis

independent variables

Wells of the cell culture experiments

dependent variables

Analytes measured using the Biolegend LEGENDplex Human CD8/NK Panel (13-plex, Catalog#740267)

control variables

Manufacturer's instructions for the Biolegend LEGENDplex Human CD8/NK Panel were followed

controls

Positive control: Not specified
Negative control: Not specified

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