OS were extracted from human milk obtained from the milk banks in San Jose, CA and Austin, TX. The extraction method was the same as in our previous publication.27 (link), 45 (link) Sodium borohydride (98%) and 2,5-dihydroxybenzoic acid (DHB) were purchased from Sigma-Aldrich (St. Louis, MO). Nonporous graphitized carbon cartridges (GCC, 150mg bed weight, 4mL cartridge volume) were bought from Alltech (Deerfield, IL). Standard HMOs were purchased from Dextra Laboratories (Earley Gate, UK). α(1-2)-Fucosidase was from EMD Calbiochem (La Jolla, CA). β(1-3)-Galactosidase was from New England Biolab (Beverly, MA). β(1-4)-Galactosidase was from ProZyme (San Leandro, CA). α(1-3,4)-Fucosidase was from Sigma-Aldrich (St. Louis, MO). All other reagents were of analytical or HPLC grade.
Protocol detail
Extraction and Analysis of Human Milk Oligosaccharides
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Other organizations : Agilent Technologies (United States), University of California, Davis
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Variable analysis
independent variables
- Milk source location (San Jose, CA and Austin, TX)
- Extraction of OS (oligosaccharides) from human milk
- Extraction method (same as in previous publications)
- Reagents used (sodium borohydride, 2,5-dihydroxybenzoic acid, nonporous graphitized carbon cartridges, standard HMOs, enzymes)
- Positive control: Standard HMOs purchased from Dextra Laboratories
- Negative controls: Not explicitly mentioned
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