Fibronectin-coated 24-well plates (Corning, New York, NY) were seeded with, 1 × 104 cells/well and incubated for 15 min, after which the non-adherent cells were washed away and the adherent cells were fixed in 4% paraformaldehyde, stained with crystal violet and counted (5 random 100× fields per well). Three independent experiments were performed, and the data are presented as the average ± SD [35 (link)].
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