Affinity Purification of Flag-Tagged Proteins
Corresponding Organization :
Other organizations : University of California, Riverside, University of North Carolina at Chapel Hill, National Institute of Environmental Health Sciences, Livestrong Foundation, Stanford University, Memorial Sloan Kettering Cancer Center, Boston University
Variable analysis
- Cell lysis procedure (as described in references 29 and 72)
- Bound proteins analyzed by western blotting
- Incubation of Anti-Flag M2-conjugated agarose beads with cell lysates overnight at 4 °C
- Extensive washing of the beads
- Positive control: Not specified
- Negative control: Not specified
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