Apoptosis and Migration Assays

TTX was diluted in culture medium to 30 μM. Staurosporine was prepared as stock in DMSO and then diluted in culture medium. In assays that exceeded 24 h, treatments were replaced daily. In vitro migration and invasion were determined using wound healing and Matrigel assays [16 (link)]. Apoptosis was determined using DeadEnd fluorometric TUNEL assay (Promega). Cell viability and proliferation were determined using trypan blue and MTT assays [16 (link)].

Free full text: Click here

Nelson M., Yang M., Millican-Slater R, & Brackenbury W.J. (2015). Nav1.5 regulates breast tumor growth and metastatic dissemination in vivo. Oncotarget, 6(32), 32914-32929.

Publication 2015

DeadEnd fluorometric TUNEL assay

Apoptosis Assays Cell viability Culture medium Dmso Fluorometric Matrigel Promega Staurosporine Trypan blue Tunel

Corresponding Organization :

Other organizations : University of York, St James's University Hospital

Top 5 similar protocols

Variable analysis

independent variables

TTX concentration (30 μM)
Staurosporine concentration (not specified)

dependent variables

Cell migration (measured using wound healing assay)
Cell invasion (measured using Matrigel assay)
Apoptosis (measured using DeadEnd fluorometric TUNEL assay)
Cell viability (measured using trypan blue assay)
Cell proliferation (measured using MTT assay)

control variables

Culture medium (used to dilute TTX and Staurosporine)
Treatment replacement (daily for assays exceeding 24 h)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!