Mouse Brain Microvascular Endothelial Cell Culture

Mouse primary brain microvascular endothelial cells (MBEC) were purchased from Cell Biologics, IL, United States (Catalog# C57-6023; Lot# 070613T2MP) and cultured according to the manual, but with no heparin (as it interferes with EV uptake Atai et al., 2013 (link); Christianson et al., 2013 (link)). Briefly, T25 flasks were pre-coated with Gelatin-based coating solution (Cell Biologics), 10⁶ cells seeded in the Endothelial Cell Medium (Cell Biologics) and passaged 1:2 upon confluence. Low passages (1–4) have been used in this study.

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Wei Z., Kale S., El Fatimy R., Rabinovsky R, & Krichevsky A.M. (2019). Co-cultures of Glioma Stem Cells and Primary Neurons, Astrocytes, Microglia, and Endothelial Cells for Investigation of Intercellular Communication in the Brain. Frontiers in Neuroscience, 13, 361.

Publication 2019

Gelatin-based coating solution Endothelial Cell Medium

Biologics Brain Cell Endothelial cell Gelatin Heparin Mouse

Corresponding Organization : Harvard University

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Variable analysis

independent variables

Culture conditions (with or without heparin)

dependent variables

Extracellular vesicle (EV) uptake

control variables

Cell type (Mouse primary brain microvascular endothelial cells)
Cell culture protocol (Seeding density, passage number, media conditions)
Cell culture substrate (Gelatin-based coating solution)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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