Thermal Analysis of Biomaterials by DSC

Differential scanning calorimetry (DSC) was carried out on DSC 2000 equipment equipment (TA Instruments, New Castle, DE, USA). Samples (5–6 mg) were placed in a standard aluminum container with a perforated lid and heated from 5 to 350 °C at a heating rate of 10 °C min⁻¹ in a nitrogen atmosphere with a set flow rate of 20 mL min⁻¹. An empty aluminum tray (<10 mg) was used as a reference probe. The experiments were performed in independent triplicates. In this analysis, the denaturation temperature (TD), the decomposition temperature (TDS), the melting temperature (Tm) and the glass transition temperature (Tg) are reported [36 (link)].