Western Blotting and Immunohistochemistry Protocol

Western blotting was performed as described previously [17 (link), 24 (link)]. The following primary antibodies were used: anti-SERPINB2 (1:1,000) (Proteintech, Wuhan, China), anti-uPAR (1:1,000) (Abcam, Cambridge, UK), anti-uPA (1:1,000) (Abcam), anti-HDAC1 (1:1,000) (Abclonal, Wuhan, China), anti-AGO2 (1:1,000) (Abclonal), anti-GST (1:1,000) (Abcam), anti-FLAG (1:1,000) (Abclonal), and anti-TDP43 (1:1,000) (Proteintech). Immunohistochemistry was performed using antibodies against TDP43 (1:200) (Proteintech), SERPINB2 (1:200) (Proteintech), and uPAR (1:200) (Abcam), as described previously [17 (link), 24 (link)].

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Xu L., Wang P., Li L., Li L., Huang Y., Zhang Y., Zheng X., Yi P., Zhang M, & Xu M. (2023). circPSD3 is a promising inhibitor of uPA system to inhibit vascular invasion and metastasis in hepatocellular carcinoma. Molecular Cancer, 22, 174.

Publication 2023

anti-uPAR anti-uPA anti-HDAC1 anti-AGO2 anti-GST anti-FLAG anti-TDP43

Ago2 Antibodies Immunohistochemistry Tdp43 Upar

Corresponding Organization : Meizhou City People's Hospital

Other organizations : West China Hospital of Sichuan University, Affiliated Hospital of North Sichuan Medical College

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Variable analysis

independent variables

Western blotting protocol
Primary antibodies used: anti-SERPINB2, anti-uPAR, anti-uPA, anti-HDAC1, anti-AGO2, anti-GST, anti-FLAG, anti-TDP43
Immunohistochemistry protocol
Antibodies used for immunohistochemistry: anti-TDP43, anti-SERPINB2, anti-uPAR

dependent variables

Protein expression levels of SERPINB2, uPAR, uPA, HDAC1, AGO2, GST, FLAG, TDP43

control variables

Western blotting and immunohistochemistry protocols were performed as described previously [17, 24]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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