MGL Expression Vector Generation

The phospho-ERK1/2 (Thr202/Tyr204), ERK1/2, phospho-AKT (Ser473), AKT, phospho-EGFR (Tyr1068) phosphor-mTOR (Ser2248) antibodies were from Cell Signaling (Danvers, MA); EGFR antibody was from Abcam (Cambridge, MA). MGL antibody was generated in our laboratory as previously described^{6 (link)}. The β-actin and α-tubulin antibodies were from Sigma (St. Louis, MO). GAPDH, vinculin, TNF-α and COX-2 antibodies were from Santa Cruz Biotechnologies (Dallas, TX). pDsRedN1-MGL vector (MGL-RFP) has been previously described^{6 (link)}. For pSRα-HA-S-tagged MGL expression vector, complete human MGL ORF was subcloned into mammalian pSRα-HA-S vector (contains HA and S tags) and DNA sequencing was performed to confirm the integrity of MGL sequence.

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Liu R., Wang X., Curtiss C., Landas S., Rong R., Sheikh M.S, & Huang Y. (2018). Monoglyceride lipase gene knockout in mice leads to increased incidence of lung adenocarcinoma. Cell Death & Disease, 9(2), 36.

Publication 2018

EGFR antibody β-actin and α-tubulin GAPDH vinculin TNF-α COX-2

Actin Antibodies Antibody Cox 2 Egfr Erk1 Gapdh Human Mammalian Mtor Phosphor Tnf α Vector Vinculin α tubulin

Corresponding Organization :

Other organizations : SUNY Upstate Medical University, University at Buffalo, State University of New York

Top 5 similar protocols

Variable analysis

independent variables

PDsRedN1-MGL vector (MGL-RFP)
PSRα-HA-S-tagged MGL expression vector

dependent variables

Phospho-ERK1/2 (Thr202/Tyr204)
ERK1/2
Phospho-AKT (Ser473)
Phospho-EGFR (Tyr1068)
Phosphor-mTOR (Ser2248)
β-actin
α-tubulin
GAPDH
Vinculin
TNF-α
COX-2

control variables

β-actin antibody
α-tubulin antibody
GAPDH antibody
Vinculin antibody

positive controls

Cell Signaling (Danvers, MA) antibodies: phospho-ERK1/2 (Thr202/Tyr204), ERK1/2, phospho-AKT (Ser473), AKT, phospho-EGFR (Tyr1068), phosphor-mTOR (Ser2248)
Abcam (Cambridge, MA) antibody: EGFR
Santa Cruz Biotechnologies (Dallas, TX) antibodies: GAPDH, vinculin, TNF-α, COX-2

negative controls

MGL antibody generated in our laboratory

Annotations

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