Antibodies for Spastin and Microtubule Proteins

Rabbit polyclonal anti-spastin (86-340) was produced as previously described [23 (link)]. Mouse monoclonal anti-spastin (6C6) and mouse monoclonal anti-Acetylated tubulin (6-11B-1) were obtained from Sigma-Aldrich. Mouse monoclonal anti-myc (4A6) was obtained from Millipore. Rabbit polyclonal anti-myc tag (ab9106), rabbit anti-GFP (ab6556) and rat anti-alpha-tubulin-tyrosinated clone YL12/2 (ab6160) were obtained from Abcam. Rat anti-RFP (5F8) was obtained from Chromotek. Goat anti-BMPR2 (EB08820) and goat anti-Kinesin-1/KIF5 (EB05492) were obtained from Everest Biotech. Rabbit polyclonal anti-ZFYVE27 (12680-1AP) and anti-IST1 (51002-1-AP) were obtained from Proteintech. Mouse monoclonal anti-TfnR (13-6800) was obtained from Invitrogen. Rabbit polyclonal anti-GAPDH (2118) was obtained from Cell Signaling Technology. Rabbit polyclonal anti-CHMP1B was produced as previously described [15 (link)]. Peroxidase conjugated secondary antibodies for western blotting were obtained from Sigma-Aldrich. Alexafluor-labelled secondary antibodies for immunofluorescence were obtained from Molecular Probes.

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Connell J.W., Allison R.J., Rodger C.E., Pearson G., Zlamalova E, & Reid E. (2019). ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic. Cellular and Molecular Life Sciences, 77(13), 2641-2658.

Publication 2019

Mouse monoclonal anti-spastin (6C6) Rat anti-RFP (5F8) anti-GAPDH (2118) Peroxidase conjugated secondary antibodies for western blotting

Alpha tubulin Antibodies Bmpr2 Clone Gapdh Goat Immunofluorescence Kinesin Molecular probes Mouse Peroxidase Rabbit Spastin Tfnr Tubulin

Corresponding Organization :

Other organizations : University of Cambridge

Top 5 similar protocols

Variable analysis

independent variables

Rabbit polyclonal anti-spastin (86-340)
Mouse monoclonal anti-spastin (6C6)
Mouse monoclonal anti-Acetylated tubulin (6-11B-1)
Mouse monoclonal anti-myc (4A6)
Rabbit polyclonal anti-myc tag (ab9106)
Rabbit anti-GFP (ab6556)
Rat anti-alpha-tubulin-tyrosinated clone YL12/2 (ab6160)
Rat anti-RFP (5F8)
Goat anti-BMPR2 (EB08820)
Goat anti-Kinesin-1/KIF5 (EB05492)
Rabbit polyclonal anti-ZFYVE27 (12680-1AP)
Rabbit polyclonal anti-IST1 (51002-1-AP)
Mouse monoclonal anti-TfnR (13-6800)
Rabbit polyclonal anti-GAPDH (2118)
Rabbit polyclonal anti-CHMP1B

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

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