Inhibiting miRNA and shRNA Targets

MiRIDIAN miRNA hairpin inhibitors and negative control #1 were obtained from Dharmacon (Lafayette, CO). These miRNA inhibitors bind to miRNAs by complementary sequences and thus block their capacity to silence mRNA target without affecting the level of miRNAs^{43 (link)}. Lentiviral particles containing shRNAs against c-Jun (TRCN0000039589, TRCN0000039590), c-Fos (TRCN0000016004, TRCN000016007) and GATA2 (TRCN0000019264, TRCN0000019265) were obtained from Sigma (St Louis, MO).

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Zhong L., Huot J, & Simard M.J. (2018). p38 activation induces production of miR-146a and miR-31 to repress E-selectin expression and inhibit transendothelial migration of colon cancer cells. Scientific Reports, 8, 2334.

Publication 2018

MiRIDIAN miRNA hairpin inhibitors c-Fos GATA2

Block C fos Gata2 Inhibitors Mirnas Mrna Shrnas

Corresponding Organization :

Other organizations : Hôtel-Dieu de Québec, Université Laval

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Variable analysis

independent variables

MiRIDIAN miRNA hairpin inhibitors and negative control #1
Lentiviral particles containing shRNAs against c-Jun, c-Fos, and GATA2

dependent variables

Silencing of mRNA targets

control variables

MiRNA levels

controls

Negative control #1

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