Isolation of Human Gingival Mesenchymal Stem Cells

Human gingival mesenchymal stem cells (hGMSCs) were isolated from the gingival tissues of three patients, in good general health conditions and without oral disease, who underwent a surgical procedure. The gingival tissues were placed in a culture dish, fragmented, washed several times in phosphate-buffered saline solution (PBS, Lonza, Basel, Switzerland) with 5% of gentamicin (Lonza) and transferred into the incubator at 37 °C in a humidified atmosphere of 5% CO₂ in air with mesenchymal stem cell growth medium-chemically defined (MSCGM-CD, Lonza). The medium was replaced every two days for approximately two weeks before the cells reached 80% confluence and were passaged in culture [26 (link)].

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Paganelli A., Diomede F., Marconi G.D., Pizzicannella J., Rajan T.S., Trubiani O, & Paganelli R. (2023). Inhibition of LPS-Induced Inflammatory Response of Oral Mesenchymal Stem Cells in the Presence of Galectin-3. Biomedicines, 11(6), 1519.

Publication 2023

PBS gentamicin MSCGM-CD

Atmosphere Cells Dish Gentamicin Gingival Human Mesenchymal stem cell Oral disease Patients Phosphate Saline solution Surgical procedure Tissues

Corresponding Organization : University of Chieti-Pescara

Other organizations : University of Modena and Reggio Emilia, Saint Camillus International University of Health and Medical Sciences

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independent variables

Isolation of human gingival mesenchymal stem cells (hGMSCs) from gingival tissues

dependent variables

Not explicitly mentioned

control variables

Gingival tissues were obtained from patients in good general health conditions and without oral disease
Gingival tissues were placed in a culture dish, fragmented, washed several times in phosphate-buffered saline solution (PBS) with 5% of gentamicin, and transferred into the incubator at 37 °C in a humidified atmosphere of 5% CO2 in air with mesenchymal stem cell growth medium-chemically defined (MSCGM-CD)
The medium was replaced every two days for approximately two weeks before the cells reached 80% confluence and were passaged in culture

controls

No positive or negative controls were explicitly mentioned in the provided information

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