Molecular Mechanisms of NF-κB Activation

Dexamethasone (D4902) (DEX) and GW7647 (G6793) (GW) were obtained from Sigma–Aldrich (St. Louis, MO, USA). Anti-GR, anti-PPARα, anti-RNA pol II and anti-p65 antibodies were obtained from Santa Cruz. Phospho-specific rabbit antibodies to p38 (Thr-180/Tyr-182), p42/44 ERK (Thr202/Tyr204), MSK1 (Thr581) and IKKα/β (Ser180/S181) were used to detect the respective phosphorylated forms and purchased from Cell Signaling. Anti-p38, anti-ERK, anti-MSK1, and anti-IκBα antibodies were purchased from Cell Signaling. Anti-tubulin and anti-actin were used as loading control and obtained from Santa Cruz. Anti-phospho-65 was obtained from Santa Cruz. Recombinant murine TNFα was produced and purified as described (30 (link)). TNFα was used at a final concentration of 2,000 IU/ml. p(IL6κB)₃50hu.IL6P-luc+ (hereafter renamed NF-κB-Luc), PPARα, GR, and 5HT7 control plasmids were described previously (21 (link), 31 (link)–33 (link)). LPS was purchased from Invivogen.

Free full text: Click here

Bougarne N., Mylka V., Ratman D., Beck I.M., Thommis J., De Cauwer L., Tavernier J., Staels B., Libert C, & De Bosscher K. (2019). Mechanisms Underlying the Functional Cooperation Between PPARα and GRα to Attenuate Inflammatory Responses. Frontiers in Immunology, 10, 1769.

Publication 2019

Anti-GR anti-PPARα anti-RNA pol II anti-p65 Anti-p38 anti-ERK anti-MSK1 anti-IκBα Anti-tubulin anti-actin LPS

Actin Anti antibodies Dexamethasone Gw7647 Ikkα Iκbα Murine Nf κb Phospho specific antibodies Plasmids Rabbit Rna pol ii Tnfα Tubulin

Corresponding Organization :

Other organizations : VIB-UGent Center for Medical Biotechnology, Ghent University, Inserm, Centre Hospitalier Universitaire de Lille, Université de Lille, Institut Pasteur de Lille, VIB-UGent Center for Inflammation Research

Top 5 similar protocols

Variable analysis

independent variables

Dexamethasone (D4902) (DEX)
GW7647 (G6793) (GW)

dependent variables

Phosphorylation of p38 (Thr-180/Tyr-182)
Phosphorylation of p42/44 ERK (Thr202/Tyr204)
Phosphorylation of MSK1 (Thr581)
Phosphorylation of IKKα/β (Ser180/S181)
NF-κB transcriptional activity

control variables

Anti-GR, anti-PPARα, anti-RNA pol II and anti-p65 antibodies
Anti-p38, anti-ERK, anti-MSK1, and anti-IκBα antibodies
Anti-tubulin and anti-actin as loading controls
Anti-phospho-65 antibody
Recombinant murine TNFα at a final concentration of 2,000 IU/ml
P(IL6κB)3 50hu.IL6P-luc+ (NF-κB-Luc), PPARα, GR, and 5HT7 control plasmids

positive controls

Recombinant murine TNFα at a final concentration of 2,000 IU/ml

negative controls

P(IL6κB)3 50hu.IL6P-luc+ (NF-κB-Luc), PPARα, GR, and 5HT7 control plasmids

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!