Measuring NfL levels in plasma samples

In the Amsterdam cohort, NfL levels were measured in both EDTA and heparin plasma using the commercially available NfL assay for SIngle MOlecule Array (SIMOA) according the kit description (NF-LIGHT™—Quanterix, Billerica, USA). For EDTA samples the result was converted by factor 1.25 in accordance to pre-analytic studies.^{11 (link)} The MGH cohort samples were analysed using the Olink® Explore 1536 platform, antibody-based multiplex technology with PCR read-out.^{9 (link)} The analytical performance of the proximity extension assay was validated for each protein assay; performance data are available at www.olink.com. Both the SIMOA and the Olink assays use the same antibodies and have been shown to be highly correlated (r = 0.9417 (Olink Target Neuro Exploratory—Olink).

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Smeele P.J., Vermunt L., Blok S., Duitman J.W., Nossent E.J., van Agtmael M.A., Heunks L.M., Horn J., Bogaard H.J, & Teunissen C.E. (2022). Neurofilament light increases over time in severe COVID-19 and is associated with delirium. Brain Communications, 4(4), fcac195.

Publication 2022

NF-LIGHT Explore 1536

Antibodies Antibody Assays Edta Factor 1 Heparin Light Multiplex pcr Plasma Protein

Corresponding Organization : Vrije Universiteit Amsterdam

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Variable analysis

independent variables

Measurement technique (SIMOA vs. Olink)

dependent variables

NfL levels

control variables

Antibodies used in both SIMOA and Olink assays
Pre-analytical conversion factor of 1.25 for EDTA samples in SIMOA assay

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