Prostate Cancer Cell Line Culture

The 22Rv1 cell line (CRL-2505) was obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA) in 2013 and cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS) and 1× (100 μg/ml) penicillin/streptomycin. The CWR-R1 late subline was generated from the CWR-R1 cell line as described (19 (link),20 (link)), and cultured in RPMI 1640 medium with 10% charcoal stripped (CS) FBS and 1× penicillin/streptomycin. The LNCaP95 cell line was provided by Jun Luo (John Hopkins University) in 2012, and cultured in phenol red-free RPMI 1640 medium (Invitrogen) supplemented with 10% FBS and 1× penicillin/streptomycin. The VCaP cell line (CRL-2876) was obtained from ATCC in 2011, and cultured in DMEM medium (Invitrogen) + 10% FBS and 1× penicillin/streptomycin.