Immunohistochemistry was performed as described previously [30 (link)]. Sections of prostates were incubated with rabbit anti-calcitonin gene-related peptide (CGRP) antibody (1:2000; Sigma-Aldrich) followed by Cy3-conjugated anti-rabbit IgG (1:200, Jackson Immunoresearch, WestGrove, PA) to immunostain peptidergic afferent (sensory) fibers. Monocytes and macrophages were stained in other prostate tissue sections using rat anti-F4/80 (1:1000; Abcam, Cambridge, MA) and Cy3-conjugated anti-rat IgG (1:200).
Protocol detail
Immunostaining Prostate Sensory Fibers
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Anti antibody
Anti igg
Calcitonin gene related peptide
Immunohistochemistry
Macrophages
Monocytes
Prostate
Rabbit
Stained tissue
Corresponding Organization :
Other organizations : University of Pittsburgh
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Variable analysis
independent variables
- Incubation of prostate sections with rabbit anti-CGRP antibody (1:2000; Sigma-Aldrich)
- Incubation of other prostate tissue sections with rat anti-F4/80 (1:1000; Abcam, Cambridge, MA)
- Immunostaining of peptidergic afferent (sensory) fibers
- Staining of monocytes and macrophages
- Concentration of Cy3-conjugated anti-rabbit IgG (1:200, Jackson Immunoresearch, WestGrove, PA)
- Concentration of Cy3-conjugated anti-rat IgG (1:200)
- Not explicitly mentioned
- Not explicitly mentioned
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