Characterization of A. rubens Luqin-Type Precursor

A transcript encoding the A. rubens luqin-type precursor (ArLQP) has been identified previously (GenBank: KT601719;^{22 (link)}). Here a cDNA containing the complete open reading frame of ArLQP was amplified by PCR from A. rubens radial nerve cord total cDNA using specific primers (see supplementary Table 1) and Q5 proofreading polymerase (NEB; Cat. No. M0491S), cloned into pCR-Blunt II TOPO vector (Invitrogen; Cat. No. K280002) and sequenced (TubeSeq service; Eurofins Genomics). The amino acid sequence of ArLQP was aligned with luqin/RYamide-type precursors from other species (see supplementary Table 2 for a list of the sequences) using MAFFT version 7 (5 iterations, substitution matrix; BLOSUM62) and highlighted using the software BOXSHADE (www.ch.embnet.org/software/BOX_form.html) with 70% conservation as the minimum for highlighting.

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Yañez-Guerra L.A., Delroisse J., Barreiro-Iglesias A., Slade S.E., Scrivens J.H, & Elphick M.R. (2018). Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome. Scientific Reports, 8, 7220.

Publication 2018

pCR-Blunt II TOPO vector TubeSeq service

Amino acid sequence Cdna Cord Luqin Primers Radial nerve Rubens Topo ii Vector

Corresponding Organization :

Other organizations : Queen Mary University of London, University of Warwick

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Variable analysis

independent variables

Specific primers used to amplify the cDNA containing the complete open reading frame of ArLQP

dependent variables

The amino acid sequence of ArLQP

control variables

Q5 proofreading polymerase used for PCR amplification
PCR-Blunt II TOPO vector used for cloning
MAFFT version 7 used for sequence alignment
BLOSUM62 substitution matrix used for sequence alignment
70% conservation as the minimum for highlighting in BOXSHADE

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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