Silencing of DDX21, Fibrillarin, and U3 snoRNA

All siRNAs and ASOs were purchased from GenePharma (Shanghai, China). For silencing DDX21, siRNA-2 5’-CGGGAAUUAAGUUCAAACGAA-3’, siRNA-a 5’-GAGACUUAAUACUGAGCAAUG-3’ and siRNA-b 5’-GGGUUGUAAUACAGUUUAUAC-3’ were used. For silencing Fibrillarin, siRNA 5’- CGAGAGAUGUGUGUUGAUA-3’ was used. For the knockdown of U3 snoRNA, U3 ASO mUmUmCmGmGTGCTCTACACmGmUmUmCmA was used as described previously [53 (link)]. N and mN are deoxynucleotide and 2′-O-methoxyethylribonucleotide, respectively. Phosphodiester backbones are phosphorothioates.

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Jiang Y., Sun S., Liu X., Su K., Zhang C., Zhang P., Zhao Z., Su Y., Wang C, & Du X. (2024). U3 snoRNA inter-regulates with DDX21 in the perichromosomal region to control mitosis. Cell Death & Disease, 15(5), 342.

Publication 2024

Corresponding Organization :

Other organizations : Peking University, Peking University Cancer Hospital

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Variable analysis

independent variables

Silencing DDX21 using siRNA-2, siRNA-a, and siRNA-b
Silencing Fibrillarin using siRNA
Knockdown of U3 snoRNA using U3 ASO

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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