E. coli Infection and Stimulation
Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College
Other organizations : Westlake University, Suzhou Institute of Systems Medicine
Variable analysis
- E. coli (JM109 strain, B528410-0001; Sanger Biotech)
- Lipopolysaccharide (LPS) from E. coli O111:B4 (L3024; Sigma)
- Recombinant human G-CSF (100 ng/ml; R&D)
- Infection of dHL-60 cells with E. coli (1 × 10^6 CFUs)
- Stimulation of dHL-60 cells with LPS (100 ng/ml)
- Treatment of dHL-60 cells with recombinant human G-CSF (100 ng/ml)
- E. coli (JM109 strain, B528410-0001; Sanger Biotech) growth as previously described [38 (link)]
- Positive control: Stimulation of dHL-60 cells with LPS (100 ng/ml)
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!