Chromogenic IHC and IF Staining

Tissues to be evaluated by chromogenic IHC detection of mCherry were counterstained with hematoxylin. All staining procedures were performed using a Leica Bond RX Automated Stainer (Leica Microsystems, Wetzlar, Germany) by HistoWiz Inc. IF staining of brain and spinal cord sections was performed as previously described [33 (link)]. Briefly, cryosections were incubated with primary antibodies overnight at 4 °C, followed by incubation with the appropriate secondary antibodies for 1 h at RT (Supplementary Table 1). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) and mounted with Vectashield® Vibrance antifade mounting media (Vector Laboratories, Newark, CA, USA).