The general procedure for ex vivo biodistribution studies was described previously39 (link), 41 (link) with minor modification in this work. Briefly, a solution of 48–50 (50 μCi / 150–200 μL) was injected into CD-1 mice via tail vein. These mice (each time point n = 4) were sacrificed at 5, 15, 30 and 60 min post tracer injection. Major organs, including whole brain, heart, liver, lung, spleen, kidneys, small intestine (including contents), muscle, testes, and blood samples were quickly harvested and weighted. The radioactivity present in these tissues was measured using 1480 Wizard gamma counter (PerkinElmer, USA), and all radioactivity measurements were automatically decay corrected based on the half-life of carbon-11. The results are expressed as the percentage of injected dose per gram of wet tissue (% ID/g).
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Ex Vivo Biodistribution of 11C-Labeled Tracers
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Corresponding Organization : National Institutes for Quantum and Radiological Science and Technology
Other organizations : Tianjin University, Scripps Research Institute, University of Oklahoma, Umeå University
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Variable analysis
independent variables
- Time post tracer injection (5, 15, 30, 60 min)
- Radioactivity present in tissues (whole brain, heart, liver, lung, spleen, kidneys, small intestine, muscle, testes, blood)
- CD-1 mouse strain
- Injection of 48-50 solution (50 μCi / 150-200 μL) via tail vein
- Wet tissue weight
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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