Exosome and Cell Protein Analysis

Protein extraction of exosomes and cells as well as Western blot analysis were performed according to our previous study [22 (link)]. The following antibodies were used: anti-Lamin B1 (#13435, 1:1000), anti-HSP 70 (#4872, 1:1000), anti-p-mTOR (#5536, 1:1000), anti-mTOR (#2983, 1:1000), anti-P-gp (#13342, 1:500) (All from Cell Signaling); anti-CD63 (D360973, 1:500) and anti-HMGA2 (D160487, 1:500) (Both from BBI) and anti-GAPDH (CW0100, 1:1000, Beijing Com Win).

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Jiang L., Zhang Y., Guo L., Liu C., Wang P, & Ren W. (2021). Exosomal microRNA-107 reverses chemotherapeutic drug resistance of gastric cancer cells through HMGA2/mTOR/P-gp pathway. BMC Cancer, 21, 1290.

Publication 2021

anti-Lamin B1 anti-HSP 70 anti-p-mTOR anti-mTOR anti-P-gp

Anti b1 Antibodies Cells Exosomes Gapdh Hmga2 Hsp 70 Lamin Lamin b1 Mtor Protein Western blot analysis

Corresponding Organization : First Affiliated Hospital of Henan University of Traditional Chinese Medicine

Other organizations : Henan University of Traditional Chinese Medicine

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