Histopathological Examination of Wound Healing

Samples were isolated from animals 3, 6, 9, 12 and 15 days post-wounding for histopathological examination. The skin specimens were immediately fixed in formal alcohol until processed^{133 (link),134 (link)}. Each specimen was then dehydrated and embedded, and thin sections (3 μm) were prepared. For immunohistochemistry, tissue sections were processed and stained with the following primary antibodies (anti-PECAM-1 and anti-CCL2) (Santa Cruz Biotechnology).

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Salah M., Badr G., Hetta H.F., Khalifa W.A, & Shoreit A.A. (2022). Fig latex inhibits the growth of pathogenic bacteria invading human diabetic wounds and accelerates wound closure in diabetic mice. Scientific Reports, 12, 21852.

Publication 2022

anti-PECAM-1

Alcohol Animals Antibodies Ccl2 Immunohistochemistry Pecam 1 Skin Thin sections Tissue

Corresponding Organization : Assiut University

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Variable analysis

independent variables

Time post-wounding (3, 6, 9, 12, and 15 days)

dependent variables

Histopathological examination of skin specimens

control variables

Tissue processing (immediate fixation in formal alcohol, dehydration, embedding, and thin sectioning at 3 μm)
Immunohistochemistry (tissue sections processed and stained with primary antibodies anti-PECAM-1 and anti-CCL2)

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