Effects of Vitamin D and BPA on Mice

The animals were randomly divided into control and treated groups 12 mice in each (six males and six females). The control groups were further divided into sham (no treatment) and vehicle (sterile corn oil), whereas the treatment group was divided into VitD (2,195 IU/kg), BPA (50 μg/kg), and BPA+VitD (50 μg/kg + 2,195 IU/kg) groups. The BPA and VitD doses were administered based on previously recommended doses [23 (link)–27 (link)]. Corn oil, VitD, and BPA were administrated daily, intraperitoneally (i.p), for six weeks. One week later, at 10.5 weeks old, animals were sacrificed, and blood and spleen samples were collected for biochemical and histological examinations (Fig 1).
The mice were administered intraperitoneally with 50 μg VitD, 2195 IU BPA/kg body weight, either alone or in combination, respectively, for six weeks.