Intracellular Arginase-1 Staining

For intracellular staining of arginase-1, cells were fixed and permeabilized with Fix/perm buffer (eBioscience), washed and then stained with anti-arginase-1 (BD biosciences), as described before (28 (link)).

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Hongo D., Tang X., Baker J., Engleman E.G, & Strober S. (2014). Requirement for Interactions of Natural Killer T Cells and Myeloid Derived Suppressor Cells for Transplantation Tolerance. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 14(11), 2467-2477.

Publication 2014

Fix/perm buffer anti-arginase-1

Arginase 1 Buffer Cells Intracellular Perm

Corresponding Organization : Stanford University

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Variable analysis

independent variables

Intracellular staining of arginase-1

dependent variables

Arginase-1 expression

control variables

Cell fixation and permeabilization with Fix/perm buffer (eBioscience)
Staining with anti-arginase-1 (BD biosciences)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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