Targeted Metabolite Analysis by HPLC-Q-TOF

Detection of metabolites was done by using a high-performance liquid chromatograph (HPLC) 1260 Infinity Series (Agilent Technologies, Santa Clara, USA) equipped with a DAD (Diode Array Detector) system (Agilent Technologies, Santa Clara, USA) and coupled to a quadrupole-time of flight (Q-TOF) mass spectrometer (Agilent Technologies, Santa Clara, USA) with a Dual ESI source (Agilent Technologies, Santa Clara, USA). HPLC, UV and MS and parameters were calibrated by the Agilent Mass Hunter Data Acquisition Software, rev. B.05.01 (Agilent Technologies, Santa Clara, USA). Chromatographic separation was performed following the method reported by Comite et al.^{56 (link)} Among the detected molecules, only those with a mass error below 10 ppm and a sufficient score were reported (identification score > 80).

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Neglia G., Cotticelli A., Vassetti A., Matera R., Staropoli A., Vinale F., Salzano A, & Campanile G. (2023). Buffalo milk and rumen fluid metabolome are significantly affected by green feed. Scientific Reports, 13, 1381.

Publication 2023

1260 Infinity Series Q-TOF) mass spectrometer Dual ESI source

Chromatographic Comite High performance liquid chromatograph

Corresponding Organization :

Other organizations : University of Naples Federico II, Institute for Sustainable Plant Protection, National Research Council

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Detection of metabolites

control variables

HPLC, UV and MS parameters calibrated by Agilent Mass Hunter Data Acquisition Software
Chromatographic separation performed following the method reported by Comite et al.

controls

Positive control: None mentioned
Negative control: None mentioned

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