Detecting ERV Envelope on Lymphocytes

ERV envelope on lymphocytes was detected as previously described (42 (link); b)using an anti-MLV envelope antibody clone 83A25 (kindly provided by Leonard Evans, NIH) (41 (link)). The following antibodies were used for staining of vaginal dendritic cells: Fixable Aqua Dead Cell Stain Kit (Thermo Fisher), CD45 (clone 30-F11, BioLegend), CD11b (clone M1/70, BioLegend), and CD11c (clone N418, BioLegend). All cells were stained in 1% BSA PBS and incubated on ice for 15 to 20 minutes. Cells were acquired on BD LSRII cytometer and analyzed by FlowJo software v8.8.7 (Tree Star, Inc.).

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Jayewickreme R., Mao T., Philbrick W., Kong Y., Treger R.S., Lu P., Rakib T., Dong H., Dang-Lawson M., Guild W.A., Lau T.J., Iwasaki A, & Tokuyama M. (2022). Endogenous Retroviruses Provide Protection Against Vaginal HSV-2 Disease. Frontiers in Immunology, 12, 758721.

Publication 2021

Fixable Aqua Dead Cell Stain Kit CD45 (clone 30-F11, CD11b (clone M1/70, CD11c (clone N418,

Anti antibody Antibodies Cd11b Cells Clone Dendritic cells Lymphocytes Tree Vaginal

Corresponding Organization : University of British Columbia

Other organizations : W. M. Keck Foundation, Howard Hughes Medical Institute

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Variable analysis

independent variables

Anti-MLV envelope antibody clone 83A25

dependent variables

ERV envelope on lymphocytes

control variables

Fixable Aqua Dead Cell Stain Kit
CD45 (clone 30-F11, BioLegend)
CD11b (clone M1/70, BioLegend)
CD11c (clone N418, BioLegend)
1% BSA PBS
Incubation on ice for 15 to 20 minutes
BD LSRII cytometer
FlowJo software v8.8.7 (Tree Star, Inc.)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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