Western Blot Analysis of Immune Regulators

Western blot analysis was performed using standard procedures. Antibodies against MAVS (1:1000, Santa Cruz), IRF3, pho-IRF3 (1:1000, Abcam), NLRC3, NLRX1 (1:2000, Ting Lab (17 (link))), TRAF3 (1:200, Santa Cruz), TRAF6, Stat1 (1:500, Santa Cruz), PCBP2 and ARCH5 (1:500, Abcam), gC1qR (1:1000, Santa Cruz), β-actin (1:200,000, Abcam) were used.

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Shao W.H., Shu D.H., Zhen Y., Hilliard B., Priest S.O., Cesaroni M., Ting J.P, & Cohen P.L. (2016). Prion-like MAVS aggregation in lupus patients associates with increased interferon-I. Arthritis & rheumatology (Hoboken, N.J.), 68(11), 2697-2707.

Publication 2016

pho-IRF3 TRAF3 TRAF6 Stat1 PCBP2 gC1qR β-actin

Actin Antibodies Irf3 Mavs Stat1 Traf3 Traf6 Western blot analysis

Corresponding Organization :

Other organizations : Temple University, University of North Carolina at Chapel Hill

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Variable analysis

independent variables

Antibodies against MAVS
Antibodies against IRF3
Antibodies against pho-IRF3
Antibodies against NLRC3
Antibodies against NLRX1
Antibodies against TRAF3
Antibodies against TRAF6
Antibodies against Stat1
Antibodies against PCBP2
Antibodies against ARCH5
Antibodies against gC1qR
Antibodies against β-actin

dependent variables

Western blot analysis results

control variables

Standard procedures for Western blot analysis

controls

No positive or negative controls were explicitly mentioned

Annotations

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