Western blot analysis was conducted following the protocol provided by Liu et al.18 (link) The antibodies used were the primary antibody, rabbit monoclonal antibody to FOXG1 (1:1000, ab196868), rabbit antibody to bcl-2–associated X protein (Bax, 1:1000, ab32503), rabbit antibody to B-cell chronic lymphocytic leukemia/lymphoma 2 (Bcl-2, 1:2000, ab182858), rabbit antibody to CCND2 (1:1000, ab207604), and rabbit antibody to glyceraldehyde-3-phosphate dehydrogenase (internal reference, ab9485, 1:2500) and the secondary antibody, horseradish peroxidase-conjugated goat anti-rabbit IgG H&L (ab97051, 1:2000, Abcam Inc., Cambridge, UK) for 1 hour.
Protocol detail
Western Blotting Antibody Analysis
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Anti igg
Antibodies
Antibody
B cell chronic lymphocytic leukemia
Bcl 2
Ccnd2
Glyceraldehyde 3 phosphate dehydrogenase
Goat
Horseradish peroxidase
Lymphoma
Monoclonal antibody
Rabbit
Western blot analysis
Corresponding Organization : Second Affiliated Hospital of Jilin University
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Variable analysis
independent variables
- Rabbit monoclonal antibody to FOXG1 (1:1000, ab196868)
- Rabbit antibody to bcl-2–associated X protein (Bax, 1:1000, ab32503)
- Rabbit antibody to B-cell chronic lymphocytic leukemia/lymphoma 2 (Bcl-2, 1:2000, ab182858)
- Rabbit antibody to CCND2 (1:1000, ab207604)
- Rabbit antibody to glyceraldehyde-3-phosphate dehydrogenase (internal reference, ab9485, 1:2500)
- Horseradish peroxidase-conjugated goat anti-rabbit IgG H&L (ab97051, 1:2000, Abcam Inc., Cambridge, UK)
- Protein expression levels of FOXG1, Bax, Bcl-2, CCND2, and glyceraldehyde-3-phosphate dehydrogenase
- Western blot analysis protocol followed the protocol provided by Liu et al.18 (link)
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