Visual function was measured with optomotor response (OMR) testing at 5, 9, 13, 17, and 21 weeks of age, as previously described37 (link)–39 using the OptoMotry system (Cerebral Mechanics, USA).40 (link) Briefly, a mouse was placed on a central pedestal in an enclosed chamber of monitors, which display rotating vertical sine wave gratings of varying spatial frequency (with contrast set at 100%) or varying grating contrast (with spatial frequency set at 0.103 cycles/degree). A trained observer marked when the characteristic head tracking movement occurred (or did not occur) through a programmed staircase method to calculate the visual threshold. Measurements from the left and right eye were averaged for a combined visual threshold score. Contrast threshold results were converted to Michelson contrast values.41 (link)
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Measuring Visual Function in Mice
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Corresponding Organization : Georgia Institute of Technology
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Variable analysis
independent variables
- Age (weeks): 5, 9, 13, 17, 21
- Visual threshold measured using optomotor response (OMR) testing
- Contrast threshold (converted to Michelson contrast values)
- Spatial frequency of rotating vertical sine wave gratings (set at 0.103 cycles/degree for contrast threshold measurements)
- Contrast of rotating vertical sine wave gratings (set at 100% for spatial frequency measurements)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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