CMM cell lines A375, SK-MEL-24, SK-MEL-28 (CRL-1619, HTB-71, HTB-72, respectively) and the embryonic kidney HEK293T (CRL-3216) cell line were purchased from ATCC; ESTDAB-049 – from the European Searchable Tumor Cell and Data Bank (Tübingen, Germany), CMM cell line MNT1 [12 (link)] was a gift from P.G. Natali, University La Sapienza, Rome, Italy. The resistant A375PR1 cell line was established from A375 as we have described [13 (link)]. MNT1-DR100 is a vemurafenib and dabrafenib resistant cell line, derived from the parental cell line MNT1, previously generated by repeated exposure to increasing concentrations of dabrafenib, a selective inhibitor of BRAFV600E [14 (link)].
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Establishment and Characterization of Melanoma Cell Lines
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Corresponding Organization : Karolinska Institutet
Other organizations : École Polytechnique Fédérale de Lausanne, Uppsala University
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Variable analysis
independent variables
- Cell lines: A375, SK-MEL-24, SK-MEL-28 (CRL-1619, HTB-71, HTB-72, respectively), HEK293T (CRL-3216), ESTDAB-049, MNT1, A375PR1, MNT1-DR100
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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