Genetic Engineering of Streptomyces sp. 4F

E. coli strain DH5α was used for cloning of plasmids with pUC replication origin (pUCori); strain EPI300 (Epicentre) was used for cloning oriV-containing plasmids; and strain ET12567 was used for conjugation with Streptomyces sp. 4F [26] (link). E. coli strains were grown in Luria-Bertani medium [27] (link), while Streptomyces sp. 4F was grown on mannitol soya flour [28] (link) plates for spore preparation and on R2YE plates [29] for actinorhodin production.
Enzymes used in this study (unless specified) were purchased from NEB. GeneRuler 1-kb DNA ladder was purchased from Thermo Fermentas and λ-HindIII ladder was from TAKARA. High fidelity DNA polymerase KOD Plus Neo was purchased from TOYOBO. The lycopene standard was purchased from Sigma-Aldrich.
Primers used in this study were listed in Table S1.