Comprehensive Antibiotic Susceptibility Profiling

For recognizing all the isolates, Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry (MALDI-TOF MS, Vitek MS, bioMérieux, France) was utilized. In accordance with the 2019 Clinical and Laboratory Standards Institute’s recommendations, 28 antibiotics were tested for their susceptibility via VITEK 2 (Card number: AST-GN13) system or Kirby-Bauer Disk Diffusion (Oxoid, UK) (CLSI) method. K. pneumoniae (ATCC700603) and Escherichia coli (ATCC25922) isolates were kept as standards to ensure quality control. Agar dilution assessment with the help of ceftazidime and cefotaxime combined with clavulanate was performed to confirm ESBL. Resistance to carbapenem (meropenem, imipenem, and ertapenem) was confirmed via disk diffusion protocol.

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Tang L., Wang H., Cao K., Li Y., Li T., Huang Y, & Xu Y. (2023). Epidemiological Features and Impact of High Glucose Level on Virulence Gene Expression and Serum Resistance of Klebsiella pneumoniae Causing Liver Abscess in Diabetic Patients. Infection and Drug Resistance, 16, 1221-1230.

Publication 2023

Vitek MS Kirby-Bauer Disk Diffusion

Agar Antibiotics Carbapenem Cefotaxime Ceftazidime Clavulanate Clinical laboratory Diffusion Dilution Ertapenem Escherichia coli Imipenem K pneumoniae Kirby bauer disk diffusion Maldi Meropenem Ms ms Susceptibility

Corresponding Organization :

Other organizations : First Affiliated Hospital of Anhui Medical University, Anhui Medical University

Top 5 similar protocols

Variable analysis

independent variables

Antibiotics tested for susceptibility

dependent variables

Antibiotic susceptibility
ESBL confirmation
Carbapenem resistance

control variables

MALDI-TOF MS for isolate recognition
VITEK 2 system or Kirby-Bauer Disk Diffusion for antibiotic susceptibility testing
Agar dilution assessment with ceftazidime and cefotaxime combined with clavulanate for ESBL confirmation
Disk diffusion protocol for carbapenem resistance confirmation

controls

K. pneumoniae (ATCC700603) and Escherichia coli (ATCC25922) isolates as standards to ensure quality control

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