RNA Extraction from Tendon Tissue

Tendon was pulverising into a powder with a dismembranator (Mikro-S; Sartorius, Melsungen, Germany) following freezing in liquid nitrogen. Immediately, 20 volumes of Tri Reagent (Ambion) was added to the powdered tendon tissue and the RNA extracted and purified as described by Peffers et al. [25 (link)] (2013). RNA was quantified by using a Nanodrop ND-100 spectrophotometer (Labtech, Uckfield, East Sussex, UK) and assessed for purity by UV absorbance measurements at 260 and 280 nm.

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Peffers M.J., Fang Y., Cheung K., Wei T.K., Clegg P.D, & Birch H.L. (2015). Transcriptome analysis of ageing in uninjured human Achilles tendon. Arthritis Research & Therapy, 17(1), 33.

Publication 2015

Mikro-S Tri Reagent Nanodrop ND-100

Nitrogen Tendon Tissue

Corresponding Organization :

Other organizations : University of Liverpool, Newcastle University, Ngee Ann Polytechnic, University College London, Royal National Orthopaedic Hospital

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Variable analysis

independent variables

Pulverizing tendon into a powder using a dismembranator (Mikro-S; Sartorius, Melsungen, Germany) following freezing in liquid nitrogen

dependent variables

RNA extracted and purified from the powdered tendon tissue
RNA quantification using a Nanodrop ND-100 spectrophotometer
RNA purity assessment by UV absorbance measurements at 260 and 280 nm

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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