Carmine Red Gut Transit Time

Carmine red, which cannot be absorbed from the lumen of the gut, was used to study total GI transit time (Kimball et al., 2005 (link)). A solution of carmine red (300 µl; 6%; Sigma-Aldrich) suspended in 0.5% methylcellulose (Sigma-Aldrich) was administered by gavage through a 21 gauge round-tip feeding needle. The time at which gavage took place was recorded as T₀. After gavage, fecal pellets were monitored at 10 min intervals for the presence of carmine red. Total GI transit time was considered as the interval between T₀ and the time of first observance of carmine red in stool.

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Li Z., Chalazonitis A., Huang Y.Y., Mann J.J., Margolis K.G., Yang Q.M., Kim D.O., Côté F., Mallet J, & Gershon M.D. (2011). Essential Roles of Enteric Neuronal Serotonin in Gastrointestinal Motility and the Development/Survival of Enteric Dopaminergic Neurons. The Journal of Neuroscience, 31(24), 8998-9009.

Publication 2011

Carmine Gavage Gi transit Methylcellulose Needle Pellets Stool

Corresponding Organization :

Other organizations : Columbia University, Pitié-Salpêtrière Hospital, Sorbonne Université, Centre National de la Recherche Scientifique

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Variable analysis

independent variables

Carmine red solution (300 µl; 6%; Sigma-Aldrich) suspended in 0.5% methylcellulose (Sigma-Aldrich)

dependent variables

Total GI transit time

control variables

Gavage through a 21 gauge round-tip feeding needle

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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