All adherent
and detached cells were collected, harvested, and then washed two
times with PBS. An Annexin V, FITC (fluorescein isothiocyanate) conjugate/propidium
iodide (PI) apoptosis detection kit was used according to the manufacturer’s
procedure (ab139418 propidium iodide flow cytometry kit/BD). After
adding the Annexin V-FITC conjugate into the labeled tube for 5 min,
a PI stain was also added and the tube was incubated for 10 min in
the dark at 37 °C. Samples were investigated with scan flow cytometry
(FAC), and the data were evaluated using the CellQuest analysis software
program (Becton–Dickinson, USA).32 (link),33 (link)
and detached cells were collected, harvested, and then washed two
times with PBS. An Annexin V, FITC (fluorescein isothiocyanate) conjugate/propidium
iodide (PI) apoptosis detection kit was used according to the manufacturer’s
procedure (ab139418 propidium iodide flow cytometry kit/BD). After
adding the Annexin V-FITC conjugate into the labeled tube for 5 min,
a PI stain was also added and the tube was incubated for 10 min in
the dark at 37 °C. Samples were investigated with scan flow cytometry
(FAC), and the data were evaluated using the CellQuest analysis software
program (Becton–Dickinson, USA).32 (link),33 (link)
