RNA-seq Analysis of A375 Cell Response

A375 cells were treated with DMSO, 4 μM sunitinib, 1 μM JQ-1, or 4 μM sunitinib + 1 μM JQ-1 for 24 h. Total RNA was extracted with MagZol and used for RNA-seq analysis. Libraries were constructed and sequenced on a BGISEQ-500RS sequencer. Only genes with at least 1 read in each of the six samples and at least 50 reads in total among all samples were retained for subsequent analyses. Differentially expressed genes were defined as those with a | log2-fold-change | > 1 and q value < 0.05.

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Zeng F., Li Y., Meng Y., Sun H., He Y., Yin M., Chen X, & Deng G. (2023). BET inhibitors synergize with sunitinib in melanoma through GDF15 suppression. Experimental & Molecular Medicine, 55(2), 364-376.

Publication 2023

Cells Dmso Genes Rna seq Sunitinib

Corresponding Organization :

Other organizations : Hunan Cancer Hospital, Xiangya Hospital Central South University, Central South University

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Variable analysis

independent variables

4 μM sunitinib
1 μM JQ-1
4 μM sunitinib + 1 μM JQ-1

dependent variables

Gene expression profiles

control variables

Time of treatment (24 h)
Cell line (A375 cells)
RNA extraction method (MagZol)
RNA sequencing platform (BGISEQ-500RS sequencer)

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