Schizonts were harvested using 60% prewarmed Percoll55 (link), washed once with prewarmed RPMI, resuspended in 5% uninfected red blood cells in prewarmed RPMI and cultured for 3 h, until parasites egressed and reinvaded. Unruptured schizonts were lysed with 5% sorbitol. The remaining culture contained young rings with a 3-h synchronicity window.
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