Cultivation of Amoeba proteus and Tetrahymena
Corresponding Organization :
Other organizations : Hokkaido University, National Institute for Basic Biology, The University of Tokyo, University of Toyama, University of Hyogo
Variable analysis
- Culture medium composition (KCl, MgSO4·7H2O, CaCl2·2H2O)
- Culture temperature (25°C)
- Feeding frequency (twice a week)
- Growth and survival of Amoeba proteus
- Culture container dimensions (300 mm × 220 mm × 50 mm)
- Culture medium for Tetrahymena pyriformis (2% w/v Bacto Proteose Peptone)
- Culture duration for Tetrahymena pyriformis (3 days)
- Starvation duration for Amoeba proteus (at least 3 days)
- Positive control: Tetrahymena pyriformis cultured in 2% w/v Bacto Proteose Peptone medium
- Negative control: Amoeba proteus starved for at least 3 days before the experiments
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