Amoeba proteus was cultured in plastic boxes (300 mm×220 mm×50 mm) filled with KCM medium (9.39 µM KCl, 32.5 µM MgSO4·7H2O, 54.4 µM CaCl2·2H2O) at 25°C and fed Tetrahymena pyriformis twice a week. T. pyriformis was cultured in 2% (w/v) Bacto Proteose Peptone medium (Becton, Dickinson and Company, USA) for 3 days. A. proteus was starved for at least 3 days before the experiments.
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