The IHC study included patients with colorectal adenocarcinoma with morphologically verified diagnosis, treated in the Department of abdominal oncology, Cancer Research Institute of Tomsk National Research Medical Center (Tomsk, Russia). The study was carried out according to Declaration of Helsinki (from 1964, revised in 1975 and 1983) and was approved by the local committee of Medical Ethics of Tomsk Cancer Research Institute; all patients signed informed consent for the study. Patients were divided as we did for TCGA cohort with the exception that the number of patients in SPARC/SPP1 group differed from S100A4 group: a) with colorectal cancer (common group for SPARC/SPP1) (N=118), b) with colon cancer (N=54), c) with rectal cancer (N=64) (Supplementary Table S2). For S100A4 group: a) with colorectal cancer (common group) (N=197), b) with colon cancer (N=89), c) with rectal cancer (N=107) (Supplementary Table S3). Patients with rectal cancer and cancer of the rectosigmoid junction received neoadjuvant chemotherapy (NAC) or chemoradiotherapy (NCRT). Five-grade Mandard Tumor Regression Grading (TRG) system was used for assessment of response in patients, where TRG1 – no residual cancer, TRG2 – residual isolated cancer cells, TRG3 – fibrosis outgrowing residual cancer, TRG4 – residual cancer outgrowing fibrosis, TRG5 – absence of regressive changes (14 (link)). All patients underwent surgical treatment. In adjuvant regime, according to indications, patients received chemotherapy under the same schemes for up to 6 months. Cases of stage IV disease were excluded.
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