UHPLC-Q-TOF/MS analysis was performed on an ACQUITY UHPLC™ system (Waters Corp. Milford, MA, United States) coupled with a Synapt G1 MS system (Waters Corp. Manchester, United Kingdom). A Waters ACQUITY UHPLC HSS T3 column (100 × 2.1 mm, 1.8 μm) was used for the analysis with the column temperature at 40°C. The mobile phases were water with 0.1% formic acid (A) and acetonitrile (B). The gradient used was as follows: 0–2 min, 5%→15% B; 2–18 min, 15%→37% B; 18–25 min, 37%→50% B; 25–27 min, 50% B; 27–28 min, 50%→5% B and 28–30 min, 5% B. The flow rate was 0.5 mL/min. The injection volume of the sample was 5 μL.
The data acquisition mode was MSE. The data were obtained at 50–1500 Da. The source temperature was 100°C, the desolvation temperature was 450 °C with desolvation gas flow 850 L/h, leucine enkephaline was used as lock mass, and the capillary voltage was 3 kV. At low CE scan, the cone voltage was 30 V for ESI, and the collision energy was 6 eV (trap) and 4 eV (transfer), while it was 40–60 eV ramp (trap) and 12 eV (transfer) for ESI− and 15–25 eV ramp (trap) and 12 eV (transfer) for ESI+. The instrument was controlled by MassLynx 4.1 software (Waters Corp.).
The data acquisition mode was MSE. The data were obtained at 50–1500 Da. The source temperature was 100°C, the desolvation temperature was 450 °C with desolvation gas flow 850 L/h, leucine enkephaline was used as lock mass, and the capillary voltage was 3 kV. At low CE scan, the cone voltage was 30 V for ESI, and the collision energy was 6 eV (trap) and 4 eV (transfer), while it was 40–60 eV ramp (trap) and 12 eV (transfer) for ESI− and 15–25 eV ramp (trap) and 12 eV (transfer) for ESI+. The instrument was controlled by MassLynx 4.1 software (Waters Corp.).
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