Polycyclic Aromatic Hydrocarbons and Omega-3 Fatty Acids Protocol

Benzo(a)pyrene (BP), benz(a)anthracene (BA), chrysene, benzo(b)fluoranthene (BbF), benzo(k)fluoranthene (BkF), and dibenz(a,h)anthracene (DBA) were procured from Sigma-Aldrich (St. Louis, MO, USA). Indeno(1,2,3-c,d)pyrene (IP) was obtained from Absolute Standards (Hamden, CT, USA). Fish oil (from menhaden) was from Sigma-Aldrich (St. Louis, MO, USA). Omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were from Cayman Chemical (Ann Arbor, MI, USA). Materials for DNA extraction [31 (link),32 (link),33 (link),34 (link)], RNA isolation [35 (link)], and ³²postlabeling methods [31 (link),36 (link),37 (link)] have been published previously.

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Zhou G., Jiang W., Xia G., Wang L., Richardson M., Chu C, & Moorthy B. (2022). Attenuation of Polycyclic Aromatic Hydrocarbon (PAH)-Mediated Pulmonary DNA Adducts and Cytochrome P450 (CYP)1B1 by Dietary Antioxidants, Omega-3 Fatty Acids, in Mice. Antioxidants, 11(1), 119.

Publication 2022

Benzo(a)pyrene ), benz(a)anthracene benzo(b)fluoranthene ), benzo(k)fluoranthene Fish oil (from menhaden) eicosapentaenoic acid (EPA) docosahexaenoic acid (DHA)

Anthracene Benzo a pyrene Benzo b fluoranthene Benzo k fluoranthene Cayman Chrysene Docosahexaenoic acid Fish oil Isolation Omega 3 fatty acids eicosapentaenoic acid Pyrene

Corresponding Organization :

Other organizations : Texas A&M University, Baylor College of Medicine

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Variable analysis

independent variables

Benzo(a)pyrene (BP)
Benz(a)anthracene (BA)
Chrysene
Benzo(b)fluoranthene (BbF)
Benzo(k)fluoranthene (BkF)
Dibenz(a,h)anthracene (DBA)
Indeno(1,2,3-c,d)pyrene (IP)
Fish oil (from menhaden)
Omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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