The apoptosis assay was performed as previously described11 (link). To evaluate apoptosis, the luminometric Caspase-Glo 3/7 Assay System (Promega, Catalogue# G8091) was performed on cultured murine RAW 264.7 macrophages, according to the manufacturer’s protocol. Cells were seeded in 96-well plates at the density of 10,000 cells per well, grown at 37 °C and serum-starved for 24 hours. Apoptosis was induced with 1 μM STS treatment for 4 hours in the presence or absence of 10 μM atorvastatin, 4 nM SHP1i, or equal concentrations of their respective controls (DMSO, SWNT). For quantification, an iD3 luminometer (Molecular Devices) was used.
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Apoptosis Assay in Murine Macrophages
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Corresponding Organization : Cardiovascular Institute of the South
Other organizations : Klinikum rechts der Isar, Michigan State University, California Institute for Regenerative Medicine
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Variable analysis
independent variables
- STS treatment at 1 μM concentration
- Atorvastatin treatment at 10 μM concentration
- SHP1i treatment at 4 nM concentration
- Apoptosis, measured using the luminometric Caspase-Glo 3/7 Assay System
- Murine RAW 264.7 macrophage cell line
- Cell seeding density of 10,000 cells per well
- Cell culture conditions at 37 °C
- Serum starvation for 24 hours
- Respective controls (DMSO, SWNT) for the treatments
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