MALDI-TOF-MS Bacterial Identification Protocol

All isolates were subjected to matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) according to the manufacturer’s protocol. Raw spectra were analysed by the MALDI Biotyper 2.0 software programme with default settings (Bruker Daltonics, Bremen, Germany). The extraction method was performed as previously described [58 (link)] on overnight colonies grown on CAB at 37°C and all isolates were tested in duplicate. The bacterial test standard (E. coli DH5 alpha, Bruker, Bremen, Germany) was used for calibration before each experiment and included in duplicate on each target plate. The mass peak profiles were matched to the reference database and a score generated based on similarity [59 (link)].

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Schmidt V.M., Williams N.J., Pinchbeck G., Corless C.E., Shaw S., McEwan N., Dawson S, & Nuttall T. (2014). Antimicrobial resistance and characterisation of staphylococci isolated from healthy Labrador retrievers in the United Kingdom. BMC Veterinary Research, 10, 17.

Publication 2014

MALDI Biotyper 2.0

Bacterial E coli Maldi

Corresponding Organization :

Other organizations : University of Liverpool, Royal Liverpool University Hospital, University of Edinburgh

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

MALDI-TOF-MS mass peak profiles

control variables

Overnight colonies grown on CAB at 37°C
Bacterial test standard (E. coli DH5 alpha, Bruker, Bremen, Germany) used for calibration before each experiment and included in duplicate on each target plate

controls

Positive control: Bacterial test standard (E. coli DH5 alpha, Bruker, Bremen, Germany)
Negative control: Not specified

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